Reference: Hettema EH, et al. (1998) The cytosolic DnaJ-like protein djp1p is involved specifically in peroxisomal protein import. J Cell Biol 142(2):421-34

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Abstract

The Saccharomyces cerevisiae DJP1 gene encodes a cytosolic protein homologous to Escherichia coli DnaJ. DnaJ homologues act in conjunction with molecular chaperones of the Hsp70 protein family in a variety of cellular processes. Cells with a DJP1 gene deletion are viable and exhibit a novel phenotype among cytosolic J-protein mutants in that they have a specific impairment of only one organelle, the peroxisome. The phenotype was also unique among peroxisome assembly mutants: peroxisomal matrix proteins were mislocalized to the cytoplasm to a varying extent, and peroxisomal structures failed to grow to full size and exhibited a broad range of buoyant densities. Import of marker proteins for the endoplasmic reticulum, nucleus, and mitochondria was normal. Furthermore, the metabolic adaptation to a change in carbon source, a complex multistep process, was unaffected in a DJP1 gene deletion mutant. We conclude that Djp1p is specifically required for peroxisomal protein import.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Hettema EH, Ruigrok CCM, Koerkamp MG, van den Berg M, Tabak HF, Distel B, Braakman I
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