Take our Survey

Reference: Jong AY, et al. (1984) The CDC8 gene of yeast encodes thymidylate kinase. J Biol Chem 259(17):11052-9

Reference Help

Abstract

Thymidylate kinase catalyzes the phosphorylation of thymidine 5'-monophosphate to thymidine 5'-diphosphate in the pathway of synthesis of dTTP from dTMP. We have purified the enzyme approximately 5000-fold from a plasmid-bearing strain of the yeast Saccharomyces cerevisiae that over produces the activity 6-fold. The protein appears homogeneous by sodium dodecyl sulfate-polyacrylamide gel analysis and has a molecular weight of 25,000. The amino acid composition and the sequence of amino acids on the NH2 terminus have been determined. Our interest in thymidylate kinase stems from the fact that R. A. Sclafani and W. Fangman (personal communication) recently presented genetic evidence that this enzyme is encoded by the CDC8 gene of yeast. In this paper, we show, by several biochemical criteria, that thymidylate kinase is the product of the CDC8 gene. First, extracts of strains bearing six different alleles of cdc8 show no thymidylate kinase activity. Secondly, strains carrying the CDC8 gene on a high-copy-number plasmid produce 6-fold higher levels of the kinase activity than does wild type. Third, the DNA sequence of the CDC8 gene reveals an open reading frame that encodes a protein with the same amino-terminal sequence as purified thymidylate kinase.

Reference Type
Journal Article
Authors
Jong AY, Kuo CL, Campbell JL
Primary Lit For
Additional Lit For
Review For

Interaction Annotations

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations

Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference