Extracellular production of glucoamylase from the yeast Saccharomyces diastaticus carrying an STA1 gene (a structural gene for the enzyme) is repressed by the mating-type constitution a/alpha. We obtained mutants that were relieved from the mating-type control of the STA1 gene expression. Tetrad analysis showed that the mutation was closely linked to MATa. The mutation was complemented by a MATa2 gene; hence, it was designated mata2. Northern blot analysis revealed that the MATa2-mediated repression occurs at the posttranscriptional level.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Reference||Annotation Extension|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Conditions||Strain||Source||Reference|