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Reference: Hocine S, et al. (2013) Single-molecule analysis of gene expression using two-color RNA labeling in live yeast. Nat Methods 10(2):119-21

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Abstract


Live-cell imaging of mRNA yields important insights into gene expression, but it has generally been limited to the labeling of one RNA species and has never been used to count single mRNAs over time in yeast. We demonstrate a two-color imaging system with single-molecule resolution using MS2 and PP7 RNA labeling. We use this methodology to measure intrinsic noise in mRNA levels and RNA polymerase II kinetics at a single gene.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, N.I.H., Extramural
Authors
Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH
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