Reference: Sainz-Polo MA, et al. (2012) Crystallization and preliminary X-ray diffraction analysis of the invertase from Saccharomyces cerevisiae. Acta Crystallogr Sect F Struct Biol Cryst Commun 68(Pt 12):1538-41

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Abstract

Saccharomyces cerevisiae invertase (ScInv) is an enzyme encoded by the SUC2 gene that releases beta-fructose from the nonreducing termini of various beta-D-fructofuranoside substrates. Its ability to produce 6-kestose by transglycosylation makes this enzyme an interesting research target for applications in industrial biotechnology. The native enzyme, which presents a high degree of oligomerization, was crystallized by vapour-diffusion methods. The crystals belonged to space group P3(1)21, with unit-cell parameters a = 268.6, b = 268.6, c = 224.4 A. The crystals diffracted to 3.3 A resolution and gave complete data sets using a synchrotron X-ray source.

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Journal Article
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Sainz-Polo MA, Lafraya A, Polo A, Marin-Navarro J, Polaina J, Sanz-Aparicio J
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