Reference: Spitale RC, et al. (2013) RNA SHAPE analysis in living cells. Nat Chem Biol 9(1):18-20

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Abstract


RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) in living cells. RNA structures in human, mouse, fly, yeast and bacterial cells are read out at single-nucleotide resolution, revealing tertiary contacts and RNA-protein interactions.

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Journal Article
Authors
Spitale RC, Crisalli P, Flynn RA, Torre EA, Kool ET, Chang HY
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