Reference: de Dios CH, et al. (2013) The transmembrane protein Opy2 mediates activation of the Cek1 MAP kinase in Candida albicans. Fungal Genet Biol 50:21-32

Reference Help

Abstract

MAPK pathways are conserved and complex mechanisms of signaling in eukaryotic cells. These pathways mediate adaptation to different stress conditions by a core kinase cascade that perceives changes in the environment by different upstream elements and mediates adaptation through transcription factors. In the present work, the transmembrane protein Opy2 has been identified and functionally characterized in Candida albicans. This protein is required to trigger Cek1 phosphorylation by different stimuli such as the resumption of growth from stationary phase or the addition of the cell wall disturbing compounds zymolyase and tunicamycin. opy2 mutants display susceptibility to cell wall disturbing compounds like Congo red. However, it does not play a role in the adaptation to high osmolarity or oxidative stress, in close contrast with the situation for the homologous protein in Saccharomyces cerevisiae. The over-expression of Opy2 in a S. cerevisiae opy2ssk1 mutant partially complemented the osmosensitivity on solid medium by a Hog1-independent mechanism as well as the abnormal morphology observed in this mutant under high osmolarity. The electrophoretic pattern of CaOpy2 tagged version in S. cerevisiae suggested similar post-translational modification in both microorganisms. This protein is also involved in pathogenesis as revealed by the fact that opy2 mutants displayed a significantly reduced virulence in the Galleria mellonella model.

Reference Type
Journal Article
Authors
de Dios CH, Roman E, Diez C, Alonso-Monge R, Pla J
Primary Lit For
Additional Lit For
Review For

Interaction Annotations

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations

Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference