The removal of Gal80 protein by gene disruption turned into efficient GAL promoter-driven heterologous gene expression under anaerobic alcoholic fermentation of Saccharomyces cerevisiae. By using lipase B from C. antarctica as a reporter, the relative strength of GAL10 promoter (P(GAL) (10) ) in Deltagal80 mutant that does not require galactose as an inducer was compared to those of ADH1, PDC1, and PGK promoters which have been known to work well anaerobically in actively fermenting yeast cells under high glucose concentration. P(GAL) (10) in the Deltagal80 mutant showed 0.8-fold (ADH1), 4-fold (PDC1), and 50-fold (PGK) in promoter strength. (c) 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.CI - (c) 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Reference||Annotation Extension|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Conditions||Strain||Source||Reference|