Reference: Ahn J, et al. (2012) GAL promoter-driven heterologous gene expression in Saccharomyces cerevisiae big up tri, opengal8 strain at anaerobic alcoholic fermentation.LID - 10.1111/j.1567-1364.2012.12009.x [doi] FEMS Yeast Res

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Abstract

The removal of Gal80 protein by gene disruption turned into efficient GAL promoter-driven heterologous gene expression under anaerobic alcoholic fermentation of Saccharomyces cerevisiae. By using lipase B from C. antarctica as a reporter, the relative strength of GAL10 promoter (P(GAL) (10) ) in Deltagal80 mutant that does not require galactose as an inducer was compared to those of ADH1, PDC1, and PGK promoters which have been known to work well anaerobically in actively fermenting yeast cells under high glucose concentration. P(GAL) (10) in the Deltagal80 mutant showed 0.8-fold (ADH1), 4-fold (PDC1), and 50-fold (PGK) in promoter strength. (c) 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.CI - (c) 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Reference Type
Journal Article
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Ahn J, Park KM, Lee H, Son YJ, Choi ES
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