Reference: During L, et al. (2012) MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. PLoS One 7(9):e44373

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Abstract

A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have been characterized. Here we show that rsc nhp6DeltaDelta mutants, deficient for the function of the chromatin remodeling factor RSC and the chromatin architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at the restrictive temperature. In addition, we demonstrate that rsc8-ts16 nhp6DeltaDelta cells contain low levels of U6 snRNA and U4/U6 di-snRNA that is further exacerbated after two hours growth at the restrictive temperature. This change in U6 snRNA and U4/U6 di-snRNA levels in rsc8-ts16 nhp6DeltaDelta cells is indicative of splicing deficient conditions. We identify MRN1 (multi-copy suppressor of rsc nhp6DeltaDelta) as a growth suppressor of rsc nhp6DeltaDelta synthetic sickness. Mrn1 is an RNA binding protein that localizes both to the nucleus and cytoplasm. Genetic interactions are observed between 2 microm-MRN1 and the splicing deficient mutants snt309Delta, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of chromatin structure in mRNA processing.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
During L, Thorsen M, Petersen DS, Koster B, Jensen TH, Holmberg S
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