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Reference: Abrams EW, et al. (2012) Dynamic Assembly of Brambleberry Mediates Nuclear Envelope Fusion during Early Development. Cell 150(3):521-32

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Abstract

To accommodate the large cells following zygote formation, early blastomeres employ modified cell divisions. Karyomeres are one such modification, mitotic intermediates wherein individual chromatin masses are surrounded by nuclear envelope; the karyomeres then fuse to form a single mononucleus. We identified brambleberry, a maternal-effect zebrafish mutant that disrupts karyomere fusion, resulting in formation of multiple micronuclei. As karyomeres form, Brambleberry protein localizes to the nuclear envelope, with prominent puncta evident near karyomere-karyomere interfaces corresponding to membrane fusion sites. brambleberry corresponds to an unannotated gene with similarity to Kar5p, a protein that participates in nuclear fusion in yeast. We also demonstrate that Brambleberry is required for pronuclear fusion following fertilization in zebrafish. Our studies provide insight into the machinery required for karyomere fusion and suggest that specialized proteins are necessary for proper nuclear division in large dividing blastomeres.CI - Copyright (c) 2012 Elsevier Inc. All rights reserved.

Reference Type
Journal Article
Authors
Abrams EW, Zhang H, Marlow FL, Kapp L, Lu S, Mullins MC
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