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Reference: Park HY, et al. (2012) An unbiased analysis method to quantify mRNA localization reveals its correlation with cell motility. Cell Rep 1(2):179-84

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Abstract

Localization of mRNA is a critical mechanism used by a large fraction of transcripts to restrict its translation to specific cellular regions. Although current high-resolution imaging techniques provide ample information, the analysis methods for localization have either been qualitative or employed quantification in nonrandomly selected regions of interest. Here, we describe an analytical method for objective quantification of mRNA localization using a combination of two characteristics of its molecular distribution, polarization and dispersion. The validity of the method is demonstrated using single-molecule FISH images of budding yeast and fibroblasts. Live-cell analysis of endogenous beta-actin mRNA in mouse fibroblasts reveals that mRNA polarization has a half-life of approximately 16 min and is cross-correlated with directed cell migration. This novel approach provides insights into the dynamic regulation of mRNA localization and its physiological roles.CI - Copyright (c) 2012 The Authors. Published by Elsevier Inc. All rights reserved.

Reference Type
Journal Article
Authors
Park HY, Trcek T, Wells AL, Chao JA, Singer RH
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