Reference: Biener R, et al. (2012) Calorimetric control of the specific growth rate during fed-batch cultures of Saccharomyces cerevisiae. J Biotechnol 160(3-4):195-201

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Abstract

The specific growth rate of a Saccharomyces cerevisiae strain with glucose as limiting C-source was estimated from the measured heat flow produced by the cells. For the cultivation a standard 30 l laboratory bioreactor was used, which was extended in such a way that heat balancing is possible. The feed rate was adjusted by a feedforward/feedback controller such that the specific growth rate was kept on the desired set-point value. On the basis of experimental investigations it was demonstrated that the specific growth rate can be controlled at a given set point value below the critical value to prevent the production of growth-inhibitory ethanol due to the Crabtree effect. With this control strategy high biomass concentrations of more than 110 g l(-1) can be obtained.

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Journal Article
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Biener R, Steinkamper A, Horn T
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