Reference: Schwalb B, et al. (2012) Measurement of genome-wide RNA synthesis and decay rates with Dynamic Transcriptome Analysis (DTA). Bioinformatics 28(6):884-5

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Abstract

Standard transcriptomics measures total cellular RNA levels. Our understanding of gene regulation would be greatly improved if we could measure RNA synthesis and decay rates on a genome-wide level. To that end, the Dynamic Transcriptome Analysis (DTA) method has been developed. DTA combines metabolic RNA labeling with standard transcriptomics to measure RNA synthesis and decay rates in a precise and non-perturbing manner. Here, we present the open source R/Bioconductor software package DTA. It implements all required bioinformatics steps that allow the accurate absolute quantification and comparison of RNA turnover.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Schwalb B, Schulz D, Sun M, Zacher B, Dumcke S, Martin DE, Cramer P, Tresch A
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