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Reference: Hombauer H, et al. (2011) Mismatch repair, but not heteroduplex rejection, is temporally coupled to DNA replication. Science 334(6063):1713-6

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Abstract

In eukaryotes, it is unknown whether mismatch repair (MMR) is temporally coupled to DNA replication and how strand-specific MMR is directed. We fused Saccharomyces cerevisiae MSH6 with cyclins to restrict the availability of the Msh2-Msh6 mismatch recognition complex to either S phase or G2/M phase of the cell cycle. The Msh6-S cyclin fusion was proficient for suppressing mutations at three loci that replicate at mid-S phase, whereas the Msh6-G2/M cyclin fusion was defective. However, the Msh6-G2/M cyclin fusion was functional for MMR at a very late-replicating region of the genome. In contrast, the heteroduplex rejection function of MMR during recombination was partially functional during both S phase and G2/M phase. These results indicate a temporal coupling of MMR, but not heteroduplex rejection, to DNA replication.

Reference Type
Journal Article | Research Support, N.I.H., Extramural
Authors
Hombauer H, Srivatsan A, Putnam CD, Kolodner RD
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