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Reference: Galdieri L, et al. (2012) Determination of histone acetylation status by chromatin immunoprecipitation. Methods Mol Biol 809:255-65

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Abstract

Histone acetylation is the most studied posttranslation modification of nucleosomes. Understanding the mechanisms involved in global and promoter-specific histone acetylation will shed light on the control of transcriptional regulation. Chromatin immunoprecipitation is a powerful technique to study protein-DNA interactions in vivo. Proteins and DNA are cross-linked with formaldehyde, cells are lysed, and DNA is sheared by sonication. Protein-DNA complexes are immunoprecipitated with antibodies specific for total and acetylated histones and the relative occupancy of acetylated and total histones at selected loci is assessed by real-time PCR of the purified DNA.

Reference Type
Journal Article
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Galdieri L, Moon J, Vancura A
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