Reference: Hurley JH, et al. (1990) Regulation of an enzyme by phosphorylation at the active site. Science 249(4972):1012-6

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Abstract

The isocitrate dehydrogenase of Escherichia coli is an example of a ubiquitous class of enzymes that are regulated by covalent modification. In the three-dimensional structure of the enzyme-substrate complex, isocitrate forms a hydrogen bond with Ser113, the site of regulatory phosphorylation. The structures of Asp113 and Glu113 mutants, which mimic the inactivation of the enzyme by phosphorylation, show minimal conformational changes from wild type, as in the phosphorylated enzyme. Calculations based on observed structures suggest that the change in electrostatic potential when a negative charge is introduced either by phosporylation or site-directed mutagenesis is sufficient to inactivate the enzyme. Thus, direct interaction at a ligand binding site is an alternative mechanism to induced conformational changes from an allosteric site in the regulation of protein activity by phosphorylation.AD -Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.FAU - Hurley, J .

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Journal Article
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Hurley JH, Dean AM, Sohl JL, Koshland DE Jr, Stroud RM
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