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Reference: Shin BS, et al. (2011) Initiation factor eIF2gamma promotes eIF2-GTP-Met-tRNA(i)(Met) ternary complex binding to the 40S ribosome.LID - 10.1038/nsmb.2133 [doi] Nat Struct Mol Biol

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Abstract

In contrast to prokaryotic elongation factor EF-Tu, which delivers aminoacyl-tRNAs to the ribosomal A-site, eukaryotic initiation factor eIF2 binds methionyl initiator transfer RNA (Met-tRNA(i)(Met)) to the P-site of the 40S ribosomal subunit. The results of directed hydroxyl radical probing experiments to map binding of Saccharomyces cerevisiae eIF2 on the ribosome and on Met-tRNA(i)(Met) revealed that eIF2gamma primarily contacts the acceptor stem of Met-tRNA(i)(Met) and identified a key binding interface between domain III of eIF2gamma and 18S rRNA helix h44 on the 40S subunit. Whereas the analogous domain III of EF-Tu contacts the T stem of tRNAs, biochemical analyses demonstrated that eIF2gamma domain III is important for ribosome, not Met-tRNA(i)(Met). Thus, despite their structural similarity, eIF2 and EF-Tu bind tRNAs in substantially different manners, and we propose that the tRNA-binding domain III of EF-Tu has acquired a new ribosome-binding function in eIF2gamma.

Reference Type
Journal Article
Authors
Shin BS, Kim JR, Walker SE, Dong J, Lorsch JR, Dever TE
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