Take our Survey

Reference: Kaufmann A and Knop M (2011) Genomic Promoter Replacement Cassettes to Alter Gene Expression in the Yeast Saccharomyces cerevisiae. Methods Mol Biol 765:275-94

Reference Help

Abstract

Promoter substitutions are frequently used to regulate the expression of genes in a specific manner such as for their conditional expression or for their overexpression. Chromosomal integration of a regulatable promoter upstream of an open reading frame (ORF) by homologous recombination using PCR-based gene targeting is straightforward and enables stable alterations of the genome. Furthermore, together with the promoter exchange, the target proteins can be tagged N-terminally with an epitope or a fluorescent protein. Expression levels can be constitutively lowered or increased by using promoters of different strengths. Reversible regulation of gene expression at the level of transcription can be achieved by using either regulatable yeast-endogenous promoters (e.g., GAL1-10) or heterogeneous promoters with synthetic transcription factors (e.g., TetO). To regulate gene expression at the translational level, insertion of tetracycline-binding aptamers into the 5' untranslated region (5' UTR) of target genes can be used.

Reference Type
Journal Article
Authors
Kaufmann A, Knop M
Primary Lit For
Additional Lit For
Review For

Interaction Annotations

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations

Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference