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Reference: Tanaka T, et al. (2011) Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast. EMBO J 30(10):2019-30

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Abstract

Genetic screening of yeast for sld (synthetic lethality with dpb11) mutations has identified replication proteins, including Sld2, -3, and -5, and clarified the molecular mechanisms underlying eukaryotic chromosomal DNA replication. Here, we report a new replication protein, Sld7, identified by rescreening of sld mutations. Throughout the cell cycle, Sld7 forms a complex with Sld3, which associates with replication origins in a complex with Cdc45, binds to Dpb11 when phosphorylated by cyclin-dependent kinase, and dissociates from origins once DNA replication starts. However, Sld7 does not move with the replication fork. Sld7 binds to the nonessential N-terminal portion of Sld3 and reduces its affinity for Cdc45, a component of the replication fork. Although Sld7 is not essential for cell growth, its absence reduces the level of cellular Sld3, delays the dissociation from origins of GINS, a component of the replication fork, and slows S-phase progression. These results suggest that Sld7 is required for the proper function of Sld3 at the initiation of DNA replication.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Tanaka T, Umemori T, Endo S, Muramatsu S, Kanemaki M, Kamimura Y, Obuse C, Araki H
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