Reference: Ma XX, et al. (2011) Structural plasticity of the thioredoxin recognition site of yeast methionine s-sulfoxide reductase mxr1. J Biol Chem 286(15):13430-7

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Abstract

The methionine-S-sulfoxide reductase MsrA catalyzes the reduction of methionine sulfoxide, a ubiquitous reaction depending on the thioredoxin system. To investigate interactions between MsrA and thioredoxin (Trx), we determined the crystal structures of yeast MsrA/Mxr1 in their reduced, oxidized and Trx2-complexed forms, at 2.03, 1.90 and 2.70 A, respectively. Comparative structure analysis revealed significant conformational changes of the three loops, which form a plastic ''cushion'' to harbor the electron donor Trx2. The flexible C-terminal loop enabled Mxr1 to access the methionine sulfoxide on various protein substrates. Moreover, the plasticity of the Trx binding site on Mxr1 provides structural insights into the recognition of diverse substrates by a universal catalytic motif of Trx.

Reference Type
Journal Article
Authors
Ma XX, Guo PC, Shi WW, Luo M, Tan XF, Chen Y, Zhou CZ
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