Reference: Ashton TM, et al. (2011) Pathways for Holliday Junction Processing during Homologous Recombination in Saccharomyces cerevisiae. Mol Cell Biol 31(9):1921-33

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Abstract

The Saccharomyces cerevisiae Rmi1 protein is a component of the highly conserved Sgs1-Top3-Rmi1 complex. Deletion of SGS1, TOP3 or RMI1 is synthetically lethal when combined with the loss of the Mus81-Mms4 or Slx1-Slx4 endonucleases, which have been implicated in Holliday junction (HJ) resolution. To investigate the causes of this synthetic lethality, we isolated a temperature sensitive mutant of RMI1, named rmi1-1. At the restrictive temperature, this mutant phenocopies an rmi1Delta strain, but behaves as wild-type at the permissive temperature. Following a transient exposure to methyl methanesulfonate, rmi1-1 mutants accumulate unprocessed homologous recombination repair (HRR) intermediates. These intermediates are slowly resolved at the restrictive temperature, revealing a redundant resolution activity when Rmi1 is impaired. This resolution depends on Mus81-Mms4, but not on either Slx1-Slx4 or another HJ resolvase, Yen1. Similar results were also observed when Top3 function was impaired. We propose that the Sgs1-Top3-Rmi1 complex constitutes the main pathway for the processing of HJ-containing HRR intermediates, but that Mus81-Mms4 can also resolve these intermediates.

Reference Type
Journal Article
Authors
Ashton TM, Mankouri HW, Heidenblut A, McHugh PJ, Hickson ID
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