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Reference: Georis I, et al. (2011) Intranuclear Function for Protein Phosphatase 2A: Pph21 and Pph22 Are Required for Rapamycin-Induced GATA Factor Binding to the DAL5 Promoter in Yeast. Mol Cell Biol 31(1):92-104

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Abstract

PP2A, a central Tor pathway phosphatase consisting of a catalytic subunit (Pph21 or Pph22), a scaffold subunit (Tpd3) and one of two regulatory subunits (Cdc55 or Rts1) has been repeatedly shown to play important roles in cytoplasmically-localized signal transduction activities. In contrast, its involvement in intra-nuclear control of messenger RNA production has heretofore not been reported. Here, we demonstrate for the first time that binding of the Nitrogen Catabolite Repression-responsive GATA transcription activators (Gln3 & Gat1) to the DAL5 promoter and DAL5 expression require Pph21/22-Tpd3-Cdc55/Rts1 in rapamycin-treated glutamine-grown cells. This conclusion is supported by the following observations: (i) Rapamycin-induced DAL5 expression along with Gln3 and Gat1 binding to the DAL5 promoter fail to occur in pph21Deltapph22Delta, tpd3Delta, and cdc55Deltarts1Delta mutants. (ii) The Pph21/22 requirement persists even when Gat1 and Gln3 are rendered constitutively nuclear thus dissociating the intra-nuclear requirement of PP2A from its partial requirement for rapamycin-induced nuclear Gat1 localization. (iii) Pph21-Myc(13) weakly associates with the DAL5 promoter in a Gat1-dependent manner, whereas similar Pph22-Myc(13) association requires both Gln3 and Gat1. Finally, we demonstrate that a pph21Deltapph22Delta is epistatic to ure2Delta for nuclear Gat1 localization in untreated glutamine-grown cells, whereas for Gln3 just the opposite occurs, i.e., the ure2Delta is epistatic to a pph21Deltapph22Delta. This final observation adds additional support to our previous conclusion that the Gln3 and Gat1 GATA factor localizations are predominantly controlled by different regulatory pathways.

Reference Type
Journal Article
Authors
Georis I, Tate JJ, Feller A, Cooper TG, Dubois E
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