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Reference: Mallory MJ, et al. (2010) The Sin3p PAH Domains Provide Separate Functions Repressing Meiotic Gene Transcription in Saccharomyces cerevisiae. Eukaryot Cell 9(12):1835-44

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Abstract


Meiotic genes in budding yeast are repressed during vegetative growth but are transiently induced during specific stages of meiosis. Sin3p represses early meiotic gene (EMG) by bridging the DNA binding protein Ume6p to the histone deacetylase Rpd3p. Sin3p contains four paired amphipathic helix (PAH) domains, one of which (PAH3) is required for repressing several genes expressed during mitotic cell division. This report examines the roles of the PAH domains in mediating EMG repression during mitotic cell division and following meiotic induction. PAH2 and PAH3 are required for mitotic EMG repression while electrophoretic mobility shift assays indicate that only PAH2 is required for stable Ume6p-promoter interaction. Unlike mitotic repression, re-establishing EMG repression following transient meiotic induction requires PAH3 and PAH4. In addition, the role of Sin3p in re-establishing repression is expanded to include additional loci that it does not control during vegetative growth. These findings indicate that mitotic and post-induction EMG repression are mediated by two separate systems that utilize different Sin3p domains.

Reference Type
Journal Article
Authors
Mallory MJ, Law MJ, Buckingham LE, Strich R
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