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Reference: Crutchfield CA, et al. (2010) Mass spectrometry-based metabolomics of yeast. Methods Enzymol 470:393-426

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Abstract


Driven by the advent of metabolomics, recent years have seen renewed interest in the investigation of yeast metabolism. Here we provide a practical guide to metabolomic analysis of yeast using liquid chromatography-mass spectrometry (LC-MS). We begin with background on LC-MS and its utility in studying yeast metabolism. We then describe key issues involved at each step of a typical yeast metabolomics experiment: in experimental design, cell culture, metabolite extraction, LC-MS, and data processing and analysis. Throughout, we highlight interdependencies between the steps that are relevant to developing an integrated workflow which effectively leverages LC-MS to reveal yeast biology.CI - Copyright (c) 2010 Elsevier Inc. All rights reserved.

Reference Type
Journal Article
Authors
Crutchfield CA, Lu W, Melamud E, Rabinowitz JD
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