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Reference: Bahmed K, et al. (2011) End-processing during non-homologous end-joining: a role for exonuclease 1. Nucleic Acids Res 39(3):970-8

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Abstract


Non-homologous end-joining (NHEJ) is a critical error-prone pathway of double strand break repair. We recently showed that tyrosyl DNA phosphodiesterase 1 (Tdp1) regulates the accuracy of NHEJ repair junction formation in yeast. We assessed the role of other enzymes in the accuracy of junction formation using a plasmid repair assay. We found that exonuclease 1 (Exo1) is important in assuring accurate junction formation during NHEJ. Like tdp1Delta mutants, exo1Delta yeast cells repairing plasmids with 5'-extensions can produce repair junctions with templated insertions. We also found that exo1Delta mutants have a reduced median size of deletions when joining DNA with blunt ends. Surprisingly, exo1Delta pol4Delta mutants repair blunt ends with a very low frequency of deletions. This result suggests that there are multiple pathways that process blunt ends prior to end-joining. We propose that Exo1 acts at a late stage in end-processing during NHEJ. Exo1 can reverse nucleotide additions occurring due to polymerization, and may also be important for processing ends to expose microhomologies needed for NHEJ. We propose that accurate joining is controlled at two steps, a first step that blocks modification of DNA ends, which requires Tdp1, and a second step that occurs after synapsis that requires Exo1.

Reference Type
Journal Article
Authors
Bahmed K, Seth A, Nitiss KC, Nitiss JL
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