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Reference: Ohnuki S, et al. (2010) High-content, image-based screening for drug targets in yeast. PLoS One 5(4):e10177

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Abstract


BACKGROUND: Drug discovery and development are predicated on elucidation of the potential mechanisms of action and cellular targets of candidate chemical compounds. Recent advances in high-content imaging techniques allow simultaneous analysis of a range of cellular events. In this study, we propose a novel strategy to identify drug targets by combining genetic screening and high-content imaging in yeast. METHODOLOGY: In this approach, we infer the cellular functions affected by candidate drugs by comparing morphologic changes induced by the compounds with the phenotypes of yeast mutants. CONCLUSIONS: Using this method and four well-characterized reagents, we successfully identified previously known target genes of the compounds as well as other genes involved with functionally related cellular pathways. This is the first demonstration of a genetic high-content assay that can be used to identify drug targets based on morphologic phenotypes of a reference mutant panel.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Ohnuki S, Oka S, Nogami S, Ohya Y
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