Reference: Huang X, et al. (2009) Soft x-ray diffraction microscopy of a frozen hydrated yeast cell. Phys Rev Lett 103(19):198101

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Abstract

We report the first image of an intact, frozen hydrated eukaryotic cell using x-ray diffraction microscopy, or coherent x-ray diffraction imaging. By plunge freezing the specimen in liquid ethane and maintaining it below -170 degrees C, artifacts due to dehydration, ice crystallization, and radiation damage are greatly reduced. In this example, coherent diffraction data using 520 eV x rays were recorded and reconstructed to reveal a budding yeast cell at a resolution better than 25 nm. This demonstration represents an important step towards high resolution imaging of cells in their natural, hydrated state, without limitations imposed by x-ray optics.

Reference Type
Journal Article | Research Support, N.I.H., Extramural | Research Support, U.S. Gov't, Non-P.H.S.
Authors
Huang X, Nelson J, Kirz J, Lima E, Marchesini S, Miao H, Neiman AM, Shapiro D, Steinbrener J, Stewart A, ... Show all
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