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Reference: Koren A, et al. (2010) MRC1-dependent scaling of the budding yeast DNA replication timing program. Genome Res 20(6):781-90

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Abstract


We describe the DNA replication timing programs of fourteen yeast mutants with an extended S phase identified by a novel genome-wide screen. These mutants are associated with the DNA replication machinery, cell-cycle control and dNTP synthesis and affect different parts of S phase. In thirteen of the mutants, origin activation time scales with the duration of S phase. A limited number of origins becomes inactive in these strains, with inactive origins characterized by small replicons and are distributed throughout S phase. In sharp contrast, cells deleted of MRC1, a gene implicated in replication fork stabilization and in the replication checkpoint pathway, maintained wild-type firing times despite over two-fold lengthening of S phase. Numerous dormant origins were activated in this mutant. Our data suggests that most perturbations that lengthen S phase affect the entire program of replication timing, rather than a specific subset of origins, maintaining the relative order of origin firing time and delaying firing with relative proportions. Mrc1 emerges as a regulator of this robustness of the replication program.

Reference Type
Journal Article
Authors
Koren A, Soifer I, Barkai N
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