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Reference: Ozaydin B and Rine J (2010) Expanded Roles of the Origin Recognition Complex in the Architecture and Function of Silenced Chromatin in Saccharomyces cerevisiae. Mol Cell Biol 30(3):626-39

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Abstract


The silenced chromatin at the cryptic mating type loci (HML and HMR) of Saccharomyces requires a cell-cycle event between early S phase and G2/M phase to achieve repression. Although DNA replication per se is not essential for silencing, mutations in many of the proteins involved in DNA replication affect silencing. Each of the four silencers, which flank the silenced loci, includes an ORC binding site (ACS). ORC directly interacts with Sir1 and recruits Sir1 to the silencers. This study describes additional roles for ORC in the architecture of silenced chromatin. Using Chromatin Immunoprecipitation (ChIP) analysis, we found that ORC physically interacts throughout the internal regions of HMR as well as with silencers. This interaction depended on the presence of Sir proteins and, in part, on the HMR-I silencer. ORC remained associated with the internal regions of HMR even when these regions were recombinationally separated from the silencers. Moreover ORC could be recruited to the silencers lacking an ACS through its Sir1 interaction.

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Journal Article
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Ozaydin B, Rine J
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