Regulation of the yeast HO promoter has been shown to require the recruitment of chromatin modifying and remodelling enzymes. Despite this relatively little is known about what changes to chromatin structure occur during the course of regulation at HO. Here we use indirect end labelling in synchronised cultures to show that chromatin structure is disrupted in a region that spans -600 bp to -1800 bp relative to the transcriptional start site. Across this region there is a loss of canonical nucleosomes and a reduction in histone DNA cross-linking as monitored by chromatin Immunoprecipitation. The ATPase Snf2 is required for these alterations, but the histone acetyltransferase Gcn5 is not. This suggests that the SWI/SNF complex is directly involved in nucleosome removal at HO. We also present evidence indicating that the histone chaperone Asf1 assists in this. These observations suggest that SWI/SNF related complexes in concert with histone chaperones act to remove histone octamers from DNA during the course of gene regulation.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Annotation Extension||Reference|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Assay||Construct||Conditions||Strain Background||Reference|