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Reference: Jeong MY, et al. (2009) A novel function of Aft1 in regulating ferrioxamine B uptake: Aft1 modulates Arn3 ubiquitination in Saccharomyces cerevisiae. Biochem J 422(1):181-91

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Abstract


Aft1 is a transcriptional activator in Saccharomyces cerevisiae that responds to iron availability and regulates the expression of genes in the iron regulon, such as FET3, FTR1, and the ARN family. Using a two-hybrid screen, we found that Aft1 physically interacts with the ferrioxamine B (FOB) transporter Arn3. This interaction modulates the ability of Arn3 to take up FOB. The interaction between Arn3 and Aft1 was confirmed by beta-galactosidase, co-immunoprecipitation, and surface plasmon resonance (SPR) assays. Truncated Aft1 interacted more strongly with Arn3 and caused a higher FOB-uptake activity than full-length Aft1. Interestingly, only full-length Aft1 induced the correct localization of Arn3 in response to FOB. Furthermore, we found Aft1 affected Arn3 ubiquitination. These results suggest that Aft1 interacts with Arn3 and may regulate the ubiquitination of Arn3 in the cytosolic compartment.

Reference Type
Journal Article
Authors
Jeong MY, Kang CM, Kim JH, Heo DH, Chang M, Baek IJ, Ro HS, Choi ID, Kim TH, Yun CW
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