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Reference: Belloch C, et al. (2009) Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii. Appl Environ Microbiol 75(8):2534-44

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Abstract

Recently, a new type of hybrids resulting from the hybridization between S. cerevisiae and S. kudriavzevii were described. These strains exhibit physiological properties of potential biotechnological interest. A preliminary characterization of these hybrids showed a trend to reduce the S. kudriavzevii fraction of the hybrid genome. We characterized the genomic constitution of several wine S. cerevisiae x S. kudriavzevii strains by using a combined approach based on the RFLP analysis of gene regions, comparative genome hybridizations with S. cerevisiae DNA arrays, ploidy analysis and gene dose determination by quantitative real-time PCR. The high similarity in the genome structure of the S. cerevisiae x S. kudriavzevii hybrids under study indicates they originated from a single hybridization event. After hybridization, the hybrid genome underwent extensive chromosomal rearrangements, including chromosome losses and the generation of chimerical chromosomes by non-reciprocal recombination between homeologous chromosomes. These non-reciprocal recombinations between homeologous chromosomes occurred in highly conserved regions, such as Ty LTRs, rRNA regions, and conserved protein-coding genes. This study supports the hypothesis that chimerical chromosomes may have been generated by a similar mechanism to the recombination-mediated chromosome loss acting during the meiosis in Saccharomyces hybrids. As a result of the selective processes acting during fermentation, hybrid genomes maintained the S. cerevisiae genome but reduced the S. kudriavzevii fraction.

Reference Type
Journal Article
Authors
Belloch C, Perez-Torrado R, Gonzalez SS, Perez-Ortin JE, Garcia-Martinez J, Querol A, Barrio E
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