Reference: Dietvorst J and Brandt A (2008) Flocculation in Saccharomyces cerevisiae is repressed by the COMPASS methylation complex during high-gravity fermentation. Yeast 25(12):891-901

Reference Help

Abstract


The significance of COMPASS on silencing of the FLO and MAL genes, located close to telomeres, was studied in different Saccharomyces cerevisiae strains that fermented high concentrations of maltose (20%) with different efficiency. In one particular fast maltose-fermenting yeast strain, with constitutive expression of FLO11, the expression of FLO1, FLO5 and FLO9 was induced during fermentation of high concentrations of maltose or glucose. In another strain the expression of mainly FLO1 was induced. Mutants of these strains with a defective COMPASS, however, formed very large aggregates of cells earlier in the fermentation and more pronounced than the wild-type. The formation of the large flocs was dependent on calcium ions and was inhibited by mannose. The flocculation displayed by mutants defective in COMPASS was due to increased amounts of FLO1, FLO5 and FLO9 transcripts. COMPASS-mediated silencing of the MAL genes was detected at the later stages of fermentation in strains that fermented high concentrations of maltose slowly and incompletely, while silencing was not detectable in strains that fermented maltose fast. Thus, COMPASS, in addition to the MAL genes, is also involved in silencing the expression of the FLO1, FLO5 and FLO9 genes. Copyright (c) 2009 John Wiley & Sons, Ltd.

Reference Type
Journal Article
Authors
Dietvorst J, Brandt A
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference