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Reference: Koschubs T, et al. (2009) Identification, structure, and functional requirement of the Mediator submodule Med7N/31. EMBO J 28(1):69-80

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Abstract

Mediator is a modular multiprotein complex required for regulated transcription by RNA polymerase (Pol) II. Here, we show that the middle module of the Mediator core contains a submodule of unique structure and function that comprises the N-terminal part of subunit Med7 (Med7N) and the highly conserved subunit Med31 (Soh1). The Med7N/31 submodule shows a conserved novel fold, with two proline-rich stretches in Med7N wrapping around the right-handed four-helix bundle of Med31. In vitro, Med7N/31 is required for activated transcription and can act in trans when added exogenously. In vivo, Med7N/31 has a predominantly positive function on the expression of a specific subset of genes, including genes involved in methionine metabolism and iron transport. Comparative phenotyping and transcriptome profiling identify specific and overlapping functions of different Mediator submodules.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Koschubs T, Seizl M, Lariviere L, Kurth F, Baumli S, Martin DE, Cramer P
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