Reference: Gardner MK, et al. (2008) Chromosome congression by Kinesin-5 motor-mediated disassembly of longer kinetochore microtubules. Cell 135(5):894-906

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Abstract

During mitosis, sister chromatids congress to the spindle equator and are subsequently segregated via attachment to dynamic kinetochore microtubule (kMT) plus ends. A major question is how kMT plus-end assembly is spatially regulated to achieve chromosome congression. Here we find in budding yeast that the widely conserved kinesin-5 sliding motor proteins, Cin8p and Kip1p, mediate chromosome congression by suppressing kMT plus-end assembly of longer kMTs. Of the two, Cin8p is the major effector and its activity requires a functional motor domain. In contrast, the depolymerizing kinesin-8 motor Kip3p plays a minor role in spatial regulation of yeast kMT assembly. Our analysis identified a model where kinesin-5 motors bind to kMTs, move to kMT plus ends, and upon arrival at a growing plus end promote net kMT plus-end disassembly. In conclusion, we find that length-dependent control of net kMT assembly by kinesin-5 motors yields a simple and stable self-organizing mechanism for chromosome congression.

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Journal Article | Research Support, N.I.H., Extramural
Authors
Gardner MK, Bouck DC, Paliulis LV, Meehl JB, O'Toole ET, Haase J, Soubry A, Joglekar AP, Winey M, Salmon ED, ... Show all
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