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Reference: Addinall SG, et al. (2008) A Genomewide Suppressor and Enhancer Analysis of cdc13-1 Reveals Varied Cellular Processes Influencing Telomere Capping in Saccharomyces cerevisiae. Genetics 180(4):2251-66

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Abstract

In Saccharomyces cerevisiae, Cdc13 binds telomeric DNA to recruit telomerase and to "cap" chromosome ends. In temperature sensitive cdc13-1 mutants telomeric DNA is degraded and cell cycle progression is inhibited. To identify novel proteins and pathways that cap telomeres, or that respond to uncapped telomeres, we combined cdc13-1 with the yeast gene deletion collection and used high-throughput spot test assays to measure growth. We identified 369 gene deletions, in eight different phenotypic classes, that reproducibly demonstrated subtle genetic interactions with the cdc13-1 mutation. As expected, we identified DNA damage checkpoint, nonsense mediated decay and telomerase components in our screen. However, we also identified genes affecting casein kinase II activity, cell polarity, mRNA degradation, mitochondrial function, phosphate transport, iron transport, protein degradation and other functions. We also identified a number of genes of previously unknown function that we term RTC, for Restriction of Telomere Capping, or MTC, for Maintenance of Telomere Capping. It seems likely that many of the newly identified pathways/processes, that affect growth of budding yeast cdc13-1 mutants, will play evolutionarily conserved roles at telomeres. The high-throughput spot testing approach we describe is generally applicable and could aid in understanding other aspects of eukaryotic cell biology.

Reference Type
Journal Article
Authors
Addinall SG, Downey M, Yu M, Zubko M, Dewar J, Leake A, Hallinan J, Shaw O, James K, Wilkinson DJ, ... Show all
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