Reference: Malagon F and Jensen TH (2008) The T body, a new cytoplasmic RNA granule in Saccharomyces cerevisiae. Mol Cell Biol 28(19):6022-32

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Abstract


A large share of mRNA processing and packaging events occurs co-transcriptionally. To explore the hypothesis that transcription defects may affect mRNA fate, we analyzed poly(A)(+) RNA distribution in Saccharomyces cerevisiae strains harboring mutations in Rpb1p, the largest subunit of RNA polymerase II. In certain rpb1 mutants, a poly(A)(+) RNA granule, distinct from any known structure, strongly accumulated in a confined space of the cytoplasm. RNA and protein expressed from Ty1 retroviral-like elements co-localized with this new granule, that we have consequently named the T-body. A visual screen revealed that deletion of most genes with proposed functions in Ty1 biology unexpectedly do not alter T-body levels. In contrast, deletion of genes encoding the Mediator transcription initiation factor subunits Srb2p and Srb5p as well as the Ty1 transcriptional regulator Spt21p, greatly enhance T-body formation. Our data disclose a new cellular body putatively involved in assembly of Ty1 particles, and suggest that the cytoplasmic fate of mRNA can be affected by transcription initiation events.

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Journal Article
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Malagon F, Jensen TH
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