Take our Survey

Reference: Iyer RS, et al. (2008) Pseudohyphal differentiation defect due to mutations in GPCR and ammonium signaling is suppressed by low glucose concentration: a possible integrated role for carbon and nitrogen limitation. Curr Genet 54(2):71-81

Reference Help

Abstract

In response to carbon and/or nitrogen limitation, diploid cells of Saccharomyces cerevisiae either sporulate or develop pseudohyphae. Although the signal transduction pathways leading to these developmental changes have been extensively studied, how nutritional signals are integrated is not clearly understood. Results of this study indicate that reducing glucose concentration from 2% (SLAD) to 0.05% (SLALD) causes an increase in the magnitude of filamentation as well as a discernible reduction in the time required for pseudohyphal development. Further, the pseudohyphal defect of gpa2, gpr1and gpa2gpr1 but not the mep2 mutant strain is overcome on SLALD. Low glucose also induced pseudohyphae in mep2gpr1 but not mep2gpa2 strain suggesting that GPR1 inhibits pseudohyphae by inhibiting GPA2 function. Accordingly, deleting GPA2 in mep2gpr1 mutant abrogated pseudohyphae formation in SLALD. Further, replenishment of glucose suppressed pseudohyphal differentiation in wild-type cells grown in SLAD medium. However, in SLALD, glucose replenishment suppressed the filamentation response of gpa2 mutants but not that of strains carrying the wild-type GPA2. Increased trehalose levels correlated with decreased pseudohyphae formation. Results of this study demonstrate that filamentation in response to nitrogen limitation occurs as glucose becomes limiting.

Reference Type
Journal Article
Authors
Iyer RS, Das M, Bhat PJ
Primary Lit For
Additional Lit For
Review For

Interaction Annotations

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations

Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations

Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference