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Reference: Chary SN, et al. (2008) Trehalose-6-phosphate synthase/phosphatase regulates cell shape and plant architecture in Arabidopsis. Plant Physiol 146(1):97-107

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Abstract

The vacuole occupies most of the volume of plant cells; thus the tonoplast marker deltaTIP-GFP delineates cell shape, for example, in epidermis. This permits rapid identification of mutants. Using this strategy, we identified the cell shape phenotype 1 (csp-1) mutant in Arabidopsis thaliana. Beyond an absence of lobes in pavement cells, phenotypes included reduced trichome branching, altered leaf serrations and stem branching, and increased stomatal density. This result from a point mutation in AtTPS6 encoding a conserved amino-terminal domain thought to catalyze trehalose-6-phosphate synthesis and a carboxy-terminal phosphatase domain is catalyzing a two-step conversion to trehalose. Expression of AtTPS6 in the yeast mutants tps1 (encoding a synthase domain) and tps2 (synthase and phosphatase domains) indicates that AtTPS6 is an active trehalose synthase. AtTPS6 fully complemented defects in csp-1. Mutations in Class I genes (AtTPS1-4) indicate a role in regulating starch storage, resistance to drought and inflorescence architecture. Class II genes (AtTPS5 - AtTPS11) encode multi-functional enzymes having synthase and phosphatase activites. We show that Class II AtATPS6 regulates plant architecture, the shape of epidermal pavement cells and the branching of trichomes. Thus, beyond a role in development, we demonstrate that the Class II gene AtTPS6 is important for controlling cellular morphogenesis.

Reference Type
Journal Article
Authors
Chary SN, Hicks GR, Choi YG, Carter D, Raikhel NV
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