Take our Survey

Reference: Deeks MJ, et al. (2007) Arabidopsis CAP1 a key regulator of actin organisation and development. J Cell Sci 120(Pt 15):2609-18

Reference Help

Abstract


Maintenance of F-actin turnover is essential for plant cell morphogenesis. Actin-binding protein mutants reveal that plants place emphasis on particular aspects of actin biochemistry distinct from animals and fungi. Here we show that mutants in CAP1, an A. thaliana member of the cyclase-associated protein family, display a phenotype that establishes CAP1 as a fundamental facilitator of actin dynamics over a wide range of plant tissues. Plants homozygous for cap1 alleles show a reduction in stature and morphogenetic disruption of multiple cell types. Pollen grains exhibit reduced germination efficiency, and cap1 pollen tubes and root hairs grow at a decreased rate and to a reduced length. Live cell imaging of growing root hairs reveals actin filament disruption and cytoplasmic disorganisation in the tip growth zone. Mutant cap1 alleles also show synthetic phenotypes when combined with mutants of the Arp2/3 complex pathway, which further suggests a contribution of CAP1 to in planta actin dynamics. In yeast, CAP interacts with adenylate cyclase in a Ras signalling cascade; but plants do not have Ras. Surprisingly, cap1 plants show disruption in plant signalling pathways required for co-ordinated organ expansion suggesting that plant CAP has evolved to attain plant-specific signalling functions.

Reference Type
Journal Article
Authors
Deeks MJ, Rodrigues C, Dimmock S, Ketelaar T, Maciver SK, Malho R, Hussey PJ
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference