Since it seems quite difficult for frozen cells to repair the damage caused by freezing, the adequate responses appear to be induced during and/or after the thawing process to recover from the damage due to freezing. In this study, the cellular events happening upon the return from freezing at -30 degrees C to a growth temperature (28 degrees C) were investigated. Yap1p, an oxidative stress-responsive transcription factor, was not activated in the thawed cells, indicating that no serious oxidative stress was generated in the frozen-thawed cells. On the other hand, Msn2p and Msn4p, general stress-responsive transcription factors, were activated in the thawed cells and caused the increased expression of a number of Msn2p/Msn4p-target genes including SOD1, SOD2, and several HSP genes. Since almost no expression of Msn2p/Msn4p-target genes was induced before thawing, these results indicate that Msn2p and Msn4p play a role during the recovery process from freezing.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Reference||Annotation Extension|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Conditions||Strain||Source||Reference|