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Reference: Bhattacharyya RP, et al. (2006) The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway. Science 311(5762):822-6

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Abstract

Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We have found that the Ste5 scaffold has a more active role in the yeast mating pathway: a fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein (MAP) kinase Fus3. The resulting form of Fus3 is partially active--it is phosphorylated on only one of two key residues in the activation loop (pTyr). Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties.

Reference Type
Journal Article
Authors
Bhattacharyya RP, Remenyi A, Good MC, Bashor CJ, Falick AM, Lim WA
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