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Reference: Girish A, et al. (2005) Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing. Bioorg Med Chem Lett 15(10):2447-51

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Abstract


One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Girish A, Sun H, Yeo DS, Chen GY, Chua TK, Yao SQ
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