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Reference: Puri N, et al. (2005) Expression of bovine F1-ATPase with functional complementation in yeast Saccharomyces cerevisiae. J Biol Chem 280(23):22418-24

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Abstract


The mitochondrial F(1)F(o) ATP synthase is a multimeric enzyme complex composed of at least 16 unique peptides with an overall molecular weight of about 600 kDa. F(1) ATPase is composed of alpha(3)beta(3)gammadeltaepsilon with an overall molecular weight of 370 kDa. The genes encoding bovine F(1) ATPase have been expressed in a quintuple yeast Saccharomyces cerevisiae deletion mutant (deltaalphabetagammadeltaepsilon). This strain expressing bovine F(1) is unable to grow on medium containing a nonfermentable carbon source (YPG) indicating that the enzyme is non-functional. However, daughter strains were easily selected for growth on YPG medium and these were evolved for improved growth on YPG medium. The evolution of the strains was presumably due to mutations, but mutations in the genes encoding the subunits of the bovine F(1)-ATPase were not required for the ability of the cell to grow on YPG medium. The bovine enzyme expressed in yeast was partially purified to a specific activity of about half that of the enzyme purified from bovine heart mitochondria. These results indicate that the molecular machinery required for the assembly of the mitochondrial ATP synthase is conserved from bovine and yeast and suggest that yeast may be useful for the expression, mutagenesis, and analysis of the mammalian F(1) or F(1)F(o) ATP synthase.

Reference Type
Journal Article
Authors
Puri N, Lai-Zhang J, Meier S, Mueller DM
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