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Reference: Salinas K, et al. (2005) Characterization and purification of Saccharomyces cerevisiae RNase MRP reveals a new unique protein component. J Biol Chem 280(12):11352-60

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Abstract

In the yeast, Saccharomyces cerevisiae, RNase MRP is an essential endoribonuclease that consists of one RNA component, and at least nine protein components. Characterization of the complex is complicated by the fact that eight of the known protein components are shared with a related endoribonuclease, RNase P. To fully characterize the RNase MRP complex we purified it to apparent homogeneity in a highly active state using tandem affinity purification. In addition to the nine known protein components, both Rpr2 and a protein encoded by the essential gene YLR145w were present in our preparations of RNase MRP. Precipitation of a tagged version of Ylr145w brought with it the RNase MRP RNA, but not the RNase P RNA. A temperature-sensitive ylr145w mutant was generated and found to exhibit a rRNA processing defect identical to that seen in other RNase MRP mutants while no defect in tRNA processing was observed. Homologs of the Ylr145w protein were found in most yeasts, fungi and Arabidopsis. Based on this evidence, we propose that YLR145w encodes a novel protein component of RNase MRP, but not RNase P. We recommend that this gene be designated RMP1, for RNase MRP Protein 1.

Reference Type
Journal Article
Authors
Salinas K, Wierzbicki S, Zhou L, Schmitt ME
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