Reference: Iben S, et al. (2002) TFIIH plays an essential role in RNA polymerase I transcription. Cell 109(3):297-306

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Abstract


TFIIH is a multisubunit protein complex that plays an essential role in nucleotide excision repair and transcription of protein-coding genes. Here, we report that TFIIH is also required for ribosomal RNA synthesis in vivo and in vitro. In yeast, pre-rRNA synthesis is impaired in TFIIH ts strains. In a mouse, part of cellular TFIIH is localized within the nucleolus and is associated with subpopulations of both RNA polymerase I and the basal factor TIF-IB. Transcription systems lacking TFIIH are inactive and exogenous TFIIH restores transcriptional activity. TFIIH is required for productive but not abortive rDNA transcription, implying a postinitiation role in transcription. The results provide a molecular link between RNA polymerase I transcription and transcription-coupled repair of active ribosomal RNA genes.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Iben S, Tschochner H, Bier M, Hoogstraten D, Hozak P, Egly JM, Grummt I
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