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Reference: Coyle JE, et al. (2002) Structure of GABARAP in two conformations: implications for GABA(A) receptor localization and tubulin binding. Neuron 33(1):63-74

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Abstract


GABARAP recognizes and binds the gamma2 subunit of the GABA(A) receptor, interacts with microtubules and the N-ethyl maleimide sensitive factor, and is proposed to function in GABA(A) receptor trafficking and postsynaptic localization. We have determined the crystal structure of human GABARAP at 1.6 A resolution. The structure comprises an N-terminal helical subdomain and a ubiquitin-like C-terminal domain. Structure-based mutational analysis demonstrates that the N-terminal subdomain is responsible for tubulin binding while the C-terminal domain contains the binding site for the GABA(A). A second GABARAP crystal form was determined at 1.9 A resolution and documents that GABARAP can self-associate in a head-to-tail manner. The structural details of this oligomerization reveal how GABARAP can both promote tubulin polymerization and facilitate GABA(A) receptor clustering.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Coyle JE, Qamar S, Rajashankar KR, Nikolov DB
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