Reference: Grant PA, et al. (1999) Expanded lysine acetylation specificity of Gcn5 in native complexes. J Biol Chem 274(9):5895-900

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Abstract


The coactivator/adaptor protein Gcn5 is a conserved histone acetyltransferase, which functions as the catalytic subunit in multiple yeast transcriptional regulatory complexes. The ability of Gcn5 to acetylate nucleosomal histones is significantly reduced relative to its activity on free histones, where it predominantly modifies histone H3 at lysine 14. However, the association of Gcn5 in multisubunit complexes potentiates its nucleosomal histone acetyltransferase activity. Here, we show that the association of Gcn5 with other proteins in two native yeast complexes, Ada and SAGA (Spt-Ada-Gcn5-acetyltransferase), directly confers upon Gcn5 the ability to acetylate an expanded set of lysines on H3. Furthermore Ada and SAGA have overlapping, yet distinct, patterns of acetylation, suggesting that the association of specific subunits determines site specificity.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Grant PA, Eberharter A, John S, Cook RG, Turner BM, Workman JL
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