SGD Paper Help


Page Contents:
Zhu G and Davis TN  (1998) The fork head transcription factor Hcm1p participates in the regulation of SPC110, which encodes the calmodulin-binding protein in the yeast spindle pole body. Biochim Biophys Acta 1448(2):236-44

Abstract: We previously identified HCM1 as a dosage-dependent suppressor of a calmodulin temperature-sensitive mutant (cmd1-1). Calmodulin performs multiple functions in yeast. Here we demonstrate that the effects of HCM1 are specific to the role of calmodulin at the spindle pole body. Overexpression of HCM1 fully suppresses the temperature sensitivity of a calmodulin mutant (cmd1-3) that only has defects in assembly of the spindle pole body but does not suppress the temperature sensitivity of a calmodulin mutant (cmd1-8) that only affects other functions of calmodulin. The DNA binding specificity of Hcm1p was determined by a selection, amplification and binding protocol. The consensus sequence for an Hcmlp binding site is WAAYAAACAAW. Mutations in the DNA binding domain of Hcm1p abolish the ability of Hcmlp to specifically recognize this binding site and abolish the ability of Hcm1p to act as a suppressor of calmodulin mutants. The promoter of SPC110 contains a match to the consensus binding site. Deletion of HCM1 does not affect the basal level of SPC110 transcription, but reduces the induction that occurs late in G1 of the cell cycle.

Status: Published Type: Journal Article PubMed ID: 9920414

Topics addressed in this paper

Number of different genes curated to this paper: 10

  • To find other papers on a gene and topic, click on the colored ball in the appropriate box.
  • displays other papers with information about that topic for that gene.
  • displays other papers in SGD that are associated with that topic.
    The topic is addressed in these papers but does not describe a specific gene or chromosomal feature.
  • To go to the Locus page for a gene, click on the gene name.
Topics Genes linked to topics
CDC31 CIK1 CMD1 ESP1 FHL1 FKH1 FKH2 HCM1 PDS1 SPC110
Additional Literature blue ball blue ball blue ball blue ball
DNA/RNA Sequence Features blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball
Fungal Related Genes/Proteins blue ball blue ball blue ball blue ball
Genetic Interactions blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Mutants/Phenotypes blue ball blue ball blue ball
Primary Literature blue ball blue ball blue ball blue ball blue ball blue ball
Protein-Nucleic Acid Interactions blue ball
Regulation of blue ball blue ball blue ball blue ball
Regulatory Role blue ball
Strains/Constructs blue ball blue ball blue ball
Substrates/Ligands/Cofactors blue ball

Author Searches

To find contact information or other publications by the authors of this paper, follow these three steps:
  1. (1) Choose an author,
  2. (2) Choose a search parameter,
  3. (3) Click to implement
Page Contents: