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Kenna MA, et al.  (1998) Invading the yeast nucleus: a nuclear localization signal at the C terminus of Ty1 integrase is required for transposition in vivo. Mol Cell Biol 18(2):1115-24

Abstract: Retrotransposon Ty1 faces a formidable cell barrier during transposition--the yeast nuclear membrane which remains intact throughout the cell cycle. We investigated the mechanism by which transposition intermediates are transported from the cytoplasm (the presumed site of Ty1 DNA synthesis) to the nucleus, where they are integrated into the genome. Ty1 integrase has a nuclear localization signal (NLS) at its C terminus. Both full-length integrase and a C-terminal fragment localize to the nucleus. C-terminal deletion mutants in Ty1 integrase were used to map the putative NLS to the last 74 amino acid residues of integrase. Mutations in basic segments within this region decreased retrotransposition at least 50-fold in vivo. Furthermore, these mutant integrase proteins failed to localize to the nucleus. Production of virus-like particles, reverse transcriptase activity, and complete in vitro Ty1 integration resembled wild-type levels, consistent with failure of the mutant integrases to enter the nucleus.

Status: Published Type: Journal Article PubMed ID: 9448009

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Number of different genes curated to this paper: 45

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Topics Genes linked to topics (#1 - 10 )
YAR009C YBL005W-B YBL100W-B YBR012W-B YCL019W YDR034C-D YDR098C-B YDR210C-D YDR210W-B YDR261C-D
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Topics Genes linked to topics (#11 - 20 )
YDR261W-B YDR316W-B YDR365W-B YER138C YER160C YFL002W-A YGR027W-B YGR038C-B YGR109W-B YGR161C-D
Cellular Location blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Strains/Constructs blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball

Topics Genes linked to topics (#21 - 30 )
YGR161W-B YHL009W-B YHR214C-B YIL080W YIL082W-A YJL113W YJR027W YJR029W YLR157C-B YLR227W-B
Cellular Location blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
Function/Process blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball blue ball
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Topics Genes linked to topics (#31 - 40 )
YLR410W-B YML039W YML045W YMR045C YMR050C YNL054W-B YNL284C-B YOL103W-B YOR142W-B YOR192C-B
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Topics Genes linked to topics (#41 - 45 )
YOR343W-B YPL257W-B YPR137C-B YPR158C-D YPR158W-B
Cellular Location blue ball blue ball blue ball blue ball blue ball
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Mutants/Phenotypes blue ball blue ball blue ball blue ball blue ball
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